TABLE 2.

Attrition of non-virus-specific T cells (Thy 1.2⁺) in LCMV carrier mice after adoptive transfer of LCMV-immune or naive splenocytes^a

Expt	Donor cell type	n	% Donor CD8+ reconstitution	% Host CD8+ reduction	Results for memory cells		Results for naive cells
					No. of CD8+ memoryb host cells (106) (% change)	% Annexin V+	No. of CD8+ naivec host cells (106) (% change)	% Annexin V+
1	Naive	2	0.5		5.6 ± 1.0	15 ± 3.1	10.2 ± 0.5	5 ± 0.8
	LCMV-immune	3	3.7	28	3.2 ± 1.0 (−43)d	26 ± 5.1	8.1 ± 1.9 (−21)	7 ± 1.2
2	Naive	2	0.6		1.1 ± 0.1	15 ± 2.0	2.2 ± 0.3	5 ± 1.7
	LCMV-immune	2	5.8	27	0.6 ± 0.1 (−45)d	37 ± 1.0	1.8 ± 0.2 (−18)	7 ± 1.1
3	Naive	2	0.6		1.5 ± 0.0	11 ± 0.5	5.3 ± 1.3	4 ± 0.7
	LCMV-immune	3	3.7	29	0.7 ± 0.3 (−53)d	21 ± 3.0	4.1 ± 1.4 (−23)	3 ± 1.6

^aLCMV carrier mice (Thy 1.2⁺), deficient in LCMV-specific T cells from birth, received 3 × 10⁷ donor splenocytes intravenously from either naive or LCMV-immune Thy 1.1⁺ mice. After 6 days, recipient mice were sacrificed and host splenocytes were analyzed using MAbs to CD8, CD44, and Thy 1.2. The percentage of donor CD8 T cells was determined using MAbs to CD8 and Thy 1.1. 

^bHost memory cells defined as CD44^hi Thy 1.2⁺ 

^cHost naive cells defined as CD44^lo Thy 1.2⁺. 

^dP < 0.05 compared to grouped control.