TABLE 4.
Parameters of IFN-induced T-cell attritiona
| Expt | Mouse type | Time and treatmente | n | Results for memory cells
|
Results for naive cells
|
||
|---|---|---|---|---|---|---|---|
| No. of memory CD8 cellsb (105) (% change) | % Annexin V+ | No. of naive CD8 cellsc (105) (% change) | % Annexin V+ | ||||
| A | 129 | Day 0 | 2 | 7.4 ± 1.4 | 7 ± 0.1 | 33.0 ± 7.4 | 2 ± 0.2 |
| Day 1 poly(I:C) | 2 | 4.0 ± 1.2 (−46)d | 30 ± 3.3d | 26.0 ± 9.4 (−21) | 5 ± 0.2d | ||
| IFN-α/β R−/− | Day 0 | 3 | 5.7 ± 0.4 | 20 ± 1.7 | 34.0 ± 1.2 | 8 ± 3.7 | |
| Day 1 poly(I:C) | 3 | 9.2 ± 5.4 (+60)d | 14 ± 4.9 | 50.0 ± 3.5 (+47)d | 6 ± 3.4 | ||
| B | C57BL6/J | Day 0 | 2 | 5.6 ± 0.1 | 17 ± 1.2 | 19.5 ± 1.4 | 4 ± 0.8 |
| Day 1 poly(I:C) | 3 | 1.8 ± 0.1 (−68)d | 35 ± 4.5d | 9.0 ± 0.5 (−55)d | 7 ± 0.2d | ||
| Perforin−/− | Day 0 | 2 | 4.7 ± 1.5 | 35 ± 3.9 | 14.6 ± 0.7 | 17 ± 6.8 | |
| Day 1 poly(I:C) | 3 | 1.8 ± 0.8 (−62)d | 45 ± 2.9d | 8.0 ± 2.9 (−45)d | 21 ± 6.3 | ||
| C | C57BL6/J | Day 1 NK1.1 depletion | 2 | 4.7 ± 0.4 | 13 ± 1.9 | 12.8 ± 1.7 | 4 ± 1.4 |
| Day 1 poly(I:C) NK1.1 depletion | 3 | 2.2 ± 0.2 (−53)d | 23 ± 2.5d | 9.7 ± 0.7 (−24) | 4 ± 0.6 | ||
| D | 129 | Day 0 | 2 | 7.6 ± 1.1 | 11 ± 3.2 | 68.0 ± 0.5 | 2 ± 0.2 |
| Day 1 poly(I:C) | 2 | 2.4 ± 2.3 (−69)d | 38 ± 0.7d | 32.0 ± 3.1 (−53) | 4 ± 0.2d | ||
| IFN-γ R−/− | Day 0 | 2 | 9.9 ± 2.4 | 19 ± 0.8 | 64.0 ± 9.9 | 4 ± 1.3 | |
| Day 1 poly(I:C) | 3 | 4.6 ± 1.2 (−54)d | 26 ± 1.7d | 43.0 ± 5.8 (−33) | 1.3 ± 0.4 | ||
| E | C57BL6/J | Day 0 | 3 | 23.0 ±0.6 | 9 ± 1.4 | 56.0 ± 12.0 | 1 ± 0.6 |
| Day 1 poly(I:C) | 3 | 5.4 ± 1.2 (−77)d | 31 ± 9.5d | 42.0 ± 0.6 (−25) | 5 ± 2.9 | ||
| gld (FasL mutant) | Day 0 | 2 | 34.0 ± 2.7 | 12 ± 4.3 | 44.0 ± 2.7 | 4 ± 1.2 | |
| Day 1 poly(I:C) | 3 | 10.0 ± 2.7 (−71)d | 31 ± 3.4d | 31.0 ± 10.0 (−30) | 9 ± 5.5 | ||
a
Experiments shown are representative experiments performed at least three times independently. Mice received 100 μg of poly(I:C) i.p. and were sacrificed after 1 day. Spleens were harvested and analyzed using flow cytometry (see Materials and Methods).
b
Host memory cells defined as CD44hi.
c
Host naive cells defined as CD44lo.
d
P < 0.05 compared to grouped control.
e
Day 0, untreated at that time.