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. 2024 Sep 18;13(18):2945. doi: 10.3390/foods13182945

Table 3.

Examples of purification and identification methodologies used in the discovery of antioxidant peptides from tilapia a.

Purification Strategy Peptide Identification Peptide Sequence Reference
GFC, RP-HPLC MS/MS

  • GYTGL

  • LGATGL

  • VLGL

 [42]
GFC, RP-HPLC LC-MS/MS

  • KAFAVIDQDKSGFIEEDELKLFLQNFSAGARAGDSDGDGKIGVDEFAALVK

  • AFAVIDQDKSGFIEEDELKLFLQNFSAGARAGDSDGDGKIGVDEFAALVK

 [48]
GFC, RPC LC–MS/MS

  • EKL

  • EKP

  • HKPA

  • ELSC

  • ALSC

  • ASLCH

  • SLCH

  • LPGYF

  • LEVPGY

 [29]
AEC, RP-HPLC MS/MS

  • DPALATEPDPMPF

 [53,54]
GFC, CEC, RP-HPLC MS/MS

  • YGDEY

 [43,46]

a AEC, anion exchange chromatography; CEC, cation exchange chromatography; GFC, gel filtration chromatography; LC-MS/MS, liquid chromatography coupled with MS/MS; MS/MS, tandem mass spectrometry; RP-HPLC, reversed-phase high-performance liquid chromatography; RPC, reversed-phase chromatography.