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Expression of junctional genes is altered under the influence of NETs. Treatment with NETs (100 ng/mL) for 96 h resulted in a significant decrease in ZO1, OCL, and CDH5 expression, whereas JAM1 and CLDN levels remained unchanged compared with the control. Data were obtained from six donor-derived cell cultures and performed in triplicate (technical); number of individual experiments (n = 3); ns: not significant; ****: p < 0.0001; data are presented as the mean ± SD.
