Table. Median lethal dose of aerosolized Burkholderia mallei ATS2021 in C57BL/6 and BALB/c mice*.
| Mice† | Day | Inhaled dose | LD50 (95% CI) | TTD median (95% CI) | TTD mean (SE) |
|---|---|---|---|---|---|
| C57BL/6 |
21 | 9.9 × 10–1 | 56.4 CFU (21.3–150.2) | >21 | >21 |
| 1.32 × 101 | >21 | 16.5 (0.7) | |||
| 1.07 × 102 | 18.0 (11.0–NC) | 15.9 (0.9) | |||
| 1.15 × 103 | 4.0 (3.0–5.0) | 4.4 (0.3) | |||
| 4.49 × 103 | 3.0 (NC) | 3.1 (0.1) | |||
| 60 |
9.9 × 10–1 | 5.8 CFU (2.0–15.1) |
>60 | 51.0 (NC) | |
| 1.32 × 101 | 41.5 (12.0– NC) | 39.3 (5.6) | |||
| 1.07 × 102 | 18.0 (11.0–26.0) | 20.9 (3.2) | |||
| 1.15 × 103 | 4.0 (3.0–5.0) | 4.4 (0.3) | |||
| 4.49 × 103 |
3.0 (NC) |
3.1 (0.1) |
|||
| BALB/c | 21 | 4.00 × 10–1 | 4.1 CFU (1.8–11.3) | >21 | 4.0 (NC) |
| 2 | >21 | 4.9 (0.1) | |||
| 2.05 × 101 | 4.0 (3.0 –4.0) | 4.3 (0.3) | |||
| 1.86 × 102 | 3.0 (NC) | 3.1 (0.1) | |||
| 9.60 × 102 | 3.0 (2.0–3.0) | 2.9 (0.1) | |||
| 60 | 4.00 × 10–1 | 4.1 CFU (1.8–11.3) | >60 | 4.0 (NC) | |
| 2 | >60 | 4.9 (0.1) | |||
| 2.05 × 101 | 4.0 (3.0–4.0) | 4.3 (0.3) | |||
| 1.86E × 102 | 3.0 (NC) | 3.1 (0.1) | |||
| 9.60 × 102 | 3.0 (2.0–3.0) | 2.9 (0.1) |
*LD50, 50% lethal dose; NC, noncalculable; TTD, time to death or euthanasia in accordance with early intervention criteria. †Female; 7–9 weeks of age at time of exposure to aerosolized bacteria.