Figure 11.

Two main structural differences of the γ2 fragment compared to the α1 and β2 fragments. (a) The γ2 homopentamer, as predicted by AlphaFold2 and visualised in ChimeraX. The 10 extra amino acid sequences that are not present in α1 and β2 subunits are highlighted in dark blue. Alignment of the (b) α1 and γ2 subunit fragments, as well as (c) β2 and γ2 fragments, were performed using Matchmaker in ChimeraX. The γ2 fragment is depicted in pink, while the α1 and β2 fragments are depicted in blue and yellow, respectively. The extra beta sheets in α1 and β2 subunits are circled and depicted in red. Root mean square deviation (RMSD) measuring similarity across all pairs in α1 and γ2 fragment alignment = 1.670 Å; RMSD across all pairs in β2 and γ2 fragment alignment = 2.528 Å.