FIG. 5.

SDS-PAGE and Western blot analysis of NTR and TR SA11 4F virus. NTR or TR SA11 4F TLPs or DLPs were purified and treated with increasing concentrations of trypsin as indicated. The proteins were separated by SDS-PAGE, transferred to nitrocellulose, and detected with a hyperimmune anti-SA11 4F TLP mouse serum (A), a VP8^∗ (amino acids 160 to 186) peptide antiserum (B and D), or monoclonal antibodies against VP5^∗ (C). The location of the individual proteins is indicated on the right of each panel. The approximate molecular weights for the new bands representing previously uncharacterized cleavage products from viruses exposed to trypsin after purification are indicated.