Figure 1.

Schematic representation of the sample preparation and capture process. Lung cancer cell lines were pre-stained and detached using Cellstripper, then spiked into lysed blood. Biotinylated anti-EpCAM antibody and biotinylated rVAR2 were added, followed by washing steps post-incubation. NC@silica-SA was introduced to bind to the biotinylated tumor cells. The samples were then processed through the FETCH system, which captured NC@silica-SA bound cells, while other cells were discarded into the waste tube. The captured samples were subsequently enumerated using a flow cytometer.