Figure 5.

Cytotoxicity and therapeutic index of selected highly bactericidal HsrA inhibitors. (A) The cytotoxicity of HsrA inhibitors I, IV, V, VIII, XI, and XII toward HeLa cells was assessed at 24 h of exposure through the PrestoBlue^TM method. Experiments were performed twice in triplicate, vertical bars represent standard deviations. (B) The 50% cytotoxic concentration (CC₅₀) was defined as the ligand concentration that reduced the viability of the DMSO (vehicle)-treated cell cultures by 50%. Therapeutic index (TI) of each compound was calculated as the ratio between CC₅₀ and the minimal inhibitory concentration (MIC).