Fig 5. Metformin lotion inhibits scar tissue formation in rat skin after 10 days of wound healing.

The results show a large gap area (black arrows in A-C) found at the wound area treated with 0% ML (yellow dash line area in A-C). However, no gap is found at the wound area treated with 6% ML (green dash line area in D-F). Some normal skin-like tissues with hair follicles (yellow arrows in F) and blood vessels (black arrows in F) form at the wound area treated with 6% ML (D-F). Few hair follicles are found in the wound area treated with 0% ML (A-C). Many fibrosis-like tissues are found in the 0% ML treated wound area (yellow dash line area in A-C). However, there are much less fibrosis-like tissues found in the 6% ML treated wound area (green dash line area in D-F) compared to 0% ML treated wound. ML: Metformin Lotion. Scale bars: 1 mm (black), 500 μm (green), and 200 μm (red). Analysis was conducted by MT staining.