Fig 7. Metformin lotion decreases collagen III levels in the wounded skin of rats after 10 days of wound healing.

The results show that 6% ML decreased collagen III expression in wounded skin areas (E-H). In contrast, wounds treated with 0% ML exhibited higher levels of collagen III expression compared to the 6% ML-treated wounds (A-D). Semi-quantification results are in agreement with these findings (I). *p < 0.001 compared to the 0% ML treated wound. ML: Metformin Lotion. Scale bars: 500 μm (white), 200 μm (yellow). Analysis was conducted using immunostaining.