Fig 8. Metformin lotion reduces α-SMA levels in the wounded skin of rats after 10 days of wound healing.

The results show that 6% ML decreases α-SMA expression in wounded skin areas (E-H). In contrast, 77% of the cells in the wounded skin areas treated with 0% ML express α-SMA (A-D, I), whereas about 16.7% of the cells in the wounded skin areas treated with 6% ML express α-SMA (E-H, I). *p < 0.05, compared to the 0% ML treated wounds. ML: Metformin Lotion. Scale bars: 500 μm (white), 200 μm (yellow). C: Merged images of A and B; G: Merged images of E and F; D: Enlarged yellow box area in C; H: Enlarged yellow box area in G. Analysis was conducted using immunostaining.