Fig 11. Metformin lotion increases collagen I expression in the wounded skin of rats after 10 days of wound healing.

The results show that 6% ML increases collagen I expression in wounded skin areas (E-H). In contrast, wounds treated with 0% ML express much lower levels of collagen I compared to 6% ML-treated wounds (A-D). Semi-quantification results confirm these findings (I). C: Merged images of A and B; G: Merged images of E and F; D: Enlarged yellow box area in C; H: Enlarged yellow box area in G. *p < 0.001, compared to the 0% ML-treated wound. ML: Metformin Lotion. Scale bars: 500 μm (white), 200 μm (yellow). Analysis was conducted using immunostaining.