FIG. 3.

Infectious center assay and flow cytometry analysis of VZV-infected DCs. Dendritic cells inoculated with VZV-infected HFF were harvested at day 4 postinoculation. CD1a⁺ sorted cells (A) or media from VZV-infected fibroblasts (B) were incubated directly on HFF monolayers. Seven days later, cell monolayers were fixed and incubated with a human polyclonal VZV immune serum, followed by an FITC-conjugated anti-human antibody. In parallel, flow cytometry analysis of VZV antigen expression was performed on DCs stripped of surface antigens. Dendritic cells were inoculated with VZV-infected (E and F) or mock-infected (C and D) fibroblasts. Four days postinoculation, VZV-infected and mock-infected DCs from unstripped (C and E) and low-pH-buffer (stripped) cultures (D and F) were immunostained for CD1a and VZV antigens and analyzed by flow cytometry.