FIG. 5.

Assessment of apoptosis and cell viability in VZV-infected DCs. DCs inoculated with uninfected fibroblasts or infected fibroblasts were harvested and stained with trypan blue or spotted onto sides for a TUNEL assay. TUNEL staining on VZV-infected DCs (A), in the presence of DNase (B), and in the absence of TdT (C) is shown. Each arrowhead indicates the cytoplasm, and each arrow indicates the nucleus of the cell. The percentages of viable cells for infected and uninfected DC culture over a 7-day culture period are shown (D).