FIG. 7.

Immunofluorescence staining and flow cytometry analysis of human T lymphocytes inoculated with VZV-infected DCs. T lymphocytes were inoculated with uninfected or VZV-infected DCs. Two days later, cell preparations from mock-infected T cells (A, C, and E) and VZV-infected T cells (B, D, and F) were stained with antibodies and fluorescent conjugates to CD3, CD4, CD8, and VZV proteins and analyzed by confocal microscopy (A and B) or flow cytometry (C to F). The arrowhead indicates VZV-antigen-specific staining, and the arrows indicate CD3-specific staining.