FIG. 4.

Lectin blotting analysis of GP64 produced by various baculoviruses. GP64 was isolated from the progeny budded virus produced by AcMNPV (lanes 1)- or AcSWT-1 (lanes 2)-infected Sf9 cells, and equivalent samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to Immobilon filters. The filters were probed with rabbit anti-GP64 antibody (Ab), RCA, or SNA. (A) Lectin blots done in the absence of competing sugars. (B) Lectin blots done in the presence of competing sugars. (C) Lectin blots of GP64 from AcSWT-1-infected Sf9 cells after pretreatment with nothing (S), buffer alone (B), PNGase-F (P), or neuraminidase (N). The arrows on the right indicate the positions of GP64 and the IgG heavy chain. The arrows on the left mark the positions of protein standards, labeled according to their molecular masses in kilodaltons.