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. 2024 Jun 28;11(33):2402066. doi: 10.1002/advs.202402066

Figure 6.

Figure 6

AhR promoted IS‐induced the suppression of mitochondrial biogenesis in mTECs or primary TECs. a) Western blot images and quantitative data of PGC1α, TFAM and TOM20 in mTECs. ** P < 0.01 versus Con group, # P < 0.05 versus IS group (n = 3). b) The measurement of OCR, mtDNA copies, and the ATP content in mTECs. ** P < 0.01 versus Con group, # P < 0.05 versus IS group (n = 3). c) Representative images of Mitotracker staining in mTECs. Scale bar, 10 µm. MiNa‐based quantification of the mitochondrial morphological parameters, including individual mitochondria, mitochondrial networks, and mitochondrial length. *** P < 0.001 versus Con group, # P < 0.05, ## P < 0.01, ### P < 0.001 versus IS group (n = 3). d) Western blot images and quantitative data of PGC1α, TFAM and TOM20 in primary TECs. ** P < 0.01 versus Cre− +Con group, # P < 0.05 versus Cre− +IS group (n = 3). e) The measurement of OCR, mtDNA copies and the ATP content in primary TECs. ** P < 0.01, *** P < 0.001 versus Cre− +Con group, # P < 0.05 versus Cre− +IS group (n = 3). f) Representative images of Mitotracker staining in primary TECs. Scale bar, 10 µm. MiNa‐based quantification of the mitochondrial morphological parameters, including individual mitochondria, mitochondrial networks, and mitochondrial length. *** P < 0.001 versus Cre− +Con group, # P < 0.05, ## P < 0.01 versus Cre− +IS group (n = 3). g) Western blot images and quantitative analysis of PGC1α, TFAM and TOM20 expressions in mTECs. * P < 0.05, ** P < 0.01 versus Ad‐Con group, # P < 0.05 versus IS+Ad‐Con group (n = 3). h) The measurement of OCR, mtDNA copies and the ATP content in mTECs. * P < 0.05, ** P < 0.01, *** P < 0.001 versus Ad‐Con group, # P < 0.05 versus IS+Ad‐Con group (n = 3). i) Representative images of Mitotracker staining in mTECs. Scale bar, 10 µm. MiNa‐based quantification of the mitochondrial morphological parameters, including individual mitochondria, mitochondrial networks, and mitochondrial length. *** P < 0.001 versus Ad‐Con group, # P < 0.05, ## P < 0.01, ### P < 0.001 versus IS+Ad‐Con group (n = 3). Data were shown as mean ± SD. Statistical analysis was performed by one‐way ANOVA with Tukey's multiple comparisons test (a‐c) and two‐way ANOVA with Tukey's multiple comparisons test (d‐i).