Figure 4.

HSF5 is required for meiotic progression beyond the pachytene stage. A) Immunofluorescence staining for γH2AX and SYCP3 in surface‐spread spermatocytes from 8‐week‐old WT and Hsf5 KO mice. No diplotene spermatocytes were detected in Hsf5 KO mice (N = 3 biologically independent WT mice and KO mice; green, γH2AX; red, SYCP3; scale bars, 10 µm). B) Immunofluorescence staining for SYCP3, H1t, and γH2AX in spread spermatocytes from 8‐week‐old WT and Hsf5 KO mice and Hsf5 KO spermatocytes arrested at late pachytene (N = 3 biologically independent WT mice and KO mice; green, γH2AX; red, SYCP3; purple, H1t; scale bars, 10 µm).