Figure 8.

Requirement of DNA binding for HSF5 to regulate meiosis‐related gene expression. A) ChIP‒PCR was used to detect HSF5‐occupied sites in the promoters of target genes in human testicular tissues. Input DNA and mouse IgG pulldowns were used as positive controls and negative controls, respectively. Three independent experiments were performed. B) ChIP‒qPCR results showing increased binding of HSF5 to gene promoter regions in the group treated with the HSF5 antibody compared to that in the group treated with IgG. Three independent experiments were performed. (Two‐sided Student's t test; *P < 0.05; error bars, mean ± SEM). C) HSF5 enhanced the transcriptional activity of target genes, as shown by luciferase reporter assays. Three independent experiments were performed. (Two‐sided Student's t test; error bars, *P < 0.05; error bars, mean ± SEM). D) HSF5 binding to a specific sequence in the proximal promoter of target genes, as determined by EMSA. Three independent experiments were performed.