Figure 9.

Loss of Hsf5 leads to impaired spermatogenesis according to RNA‐seq analysis. A) GO analysis showed that the genes with diminished expression in the testes of Hsf5 KO mice were mostly involved in spermatogenesis. B) KEGG analysis indicated enrichment of the pathway involved in transcriptional regulation. C) Connection network of downregulated genes participating in meiotic progression in Hsf5 KO mice. D) Overlap between downregulated genes in Hsf5 KO mice and HSF5‐bound genes. E) GO analysis of the overlapping genes. F) PPI analysis showing the interaction network formed by Sycp1, Sycp2, Syce3, Tex15, Mdc1, Hfm1, Mlh3, and Msh5.