FIG. 2.

BRLF1 interacts with the amino- and carboxy-terminal regions of CBP in vitro. (A) Schematic diagram of GST-CBP fusion constructs. Regions implicated in CBP-protein interactions are indicated. The five CBP fragments fused in frame with GST are indicated below the GST-CBP construct (29). HAT, histone acetyltransferase. (B) Five microliters of [³⁵S]methionine-labeled in vitro-translated BRLF1 was incubated with GST alone (lane 2), GST-BRLF1 (lane 3), or GST-CBP constructs (lanes 4 to 8) bound to glutathione beads. Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by autoradiography. A direct load of in vitro-translated BRLF1 (2.5 μl) is shown in lane 1.