Table 2.

Primer pairs and PCR conditions used for molecular analyses of strains.

DNA-Region 1	Primer Pairs 1	PCR Conditions	Primer Reference
ITS	ITS-1F + ITS4	See Bien et al. (2020) [48]	Gardes & Bruns (1993) [49], White et al. (1990) [50]
LSU	LROR + LR5	See Paulin & Harrington (2000) [51]	Rehner & Samuels (1994), Vilgalys & Hester (1990) [46,47]
RPB2	RPB2-5F2 + RPB2-7cR	See Braun et al. (2018) for species of Amycosphaerella [52]; see Tanney & Seifert (2018) for species of Phacidiopycnis [53]	Liu et al. (1999) [54]
EF-1α	EF1-983F + EF1-2218R	See Arnold et al. (2021) for species of Coniochaeta [55]	Rehner & Buckley (2005) [56]
TUB	T1 + Bt-2b	See Liu et al. (2019) for species of Pestalotiopsis [54]	Glass & Donaldson (1995), O’Donnell & Cigelnik (1997) [57,58]

¹ ITS: internal transcribed spacers and intervening 5.8S nrDNA; LSU: large subunit (28S) of the nrRNA gene operon; RPB2: partial DNA-directed RNA polymerase II second-largest subunit gene; EF-1α: partial translation elongation factor 1-alpha gene; TUB: partial beta-tubulin gene.