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. 2024 Sep 12;13(9):791. doi: 10.3390/pathogens13090791

Figure 2.

Figure 2

Experimental Validation of tRF5-GluCTC-PABPC1 interaction. (A) The presence of PABPC1 in the pulldown complex of Bio-tRF5-GluCTC was revealed by western blot using an anti-PABPC1 antibody. (B) The schematic workflow used to detect RSV-induced tRF5-GluCTC in the PABPC1 pulldown complex by qRT-PCR. (C) tRF5-GluCTC in the PABPC1 pulldown complex was quantified by qRT-PCR, and western blots were performed to validate proper PABPC1 immunoprecipitation and PABPC1 input. ** p ≤ 0.01, compared to the igG RSV group (upper panel of (C)), or compared between the first and third columns or the second and fourth columns (lower panel of (C)).