Table 1.
Primer design for qRT-PCR and oligo information for pull-down experiments.
| Target | Primer | Sequence (5′-3′) |
|---|---|---|
| PABPC1 | Forward primer | GCCAGTACGCATCATGTGGTCTC |
| Reverse primer | CATACAGTGCTTTATTATCAATGG | |
| RSV N | RT primer | CTGCGATGAGTGGCAGGCTTTTTTTTTTTTAACTYAAAGCTC |
| Forward primer | ACTACAGTGTATTAGACTTRACAGCAGAAG | |
| Reverse primer | CTGCGATGAGTGGCAGGC | |
| RSV L | RT primer | CTGCGATGAGTGGCAGGCTTTTTTTTTTTTCATTATTCATTATG |
| Forward primer | CTTACCTAAGTGAATTGTTAAACAGCTTGAC | |
| Reverse primer | CTGCGATGAGTGGCAGGC | |
| RSV (-) | RT primer | CTGCGATGAGTGGCAGGCACTACAGTGTATTAGACTTRACAGCAGAAG |
| Forward primer | GCATCTTCTCCATGRAATTCAGG | |
| Reverse primer | CTGCGATGAGTGGCAGGC | |
| tRF5-GluCTC | RT primer | CGTCGGACTGTAGAACTCTCAAAGC |
| Foward primer | TCCCTGGTGGTCTAGTG | |
| Reverse primer | CGTCGGACTGTAGAACTCTCAAAGC | |
| Bio-tRF5-GluCTC | UCCCUGGUGGUCUAGUGGUUAGGAUUCGG-Biotin | |
| Bio-tRF5-CN | AGGUCCAACUAAAUCACUAAUAAUAAACCGC-Biotin |