Figure 8.

Transactivation assays for the determination of AR agonist/antagonist activity induced by the nine minor cannabinoids, CBG (A), CBDV (B), CBN (C), CBDB (D), CBDA (E), CBDM (F), CBCA (G), CBGVA (H), and CBC (I). CHO-K1 cells were treated with the cannabinoids (1, 5, and 10 μM) in the absence (AR agonism) or presence (AR antagonism) of R1881 (0.1 nM) for 24 h. Results are presented as the mean ± SEM of at least three independent experiments performed in triplicate. Statistically significant differences between the control (cells not exposed to cannabinoids), set as 1, and cells not treated with R1881 are expressed as § (p < 0.05), §§ (p < 0.01), and §§§ (p < 0.001), while the differences between the control and cells treated with cannabinoids in the presence of R1881 are expressed as ££ (p < 0.01) and £££ (p < 0.001).