Table 4.

Biodistribution of [^99mTc]Tc-DT11+[^99mTc]Tc-DB7 in SCID mice bearing PC-3 xenografts at 4 h pi (controls, Entresto^®-treated, and block 1 (for NTS₁R), 2 (for GRPR) and 3 (for both NTS₁R and GRPR); data are expressed as average %IA/g values ± sd, n = 4.

Organs/Tissues	Controls	Entresto® 1	Block 1 2	Block 2 3	Block 3 4
Blood	1.01 ± 0.29	0.72 ± 0.13	1.08 ± 0.13	1.64 ± 0.52	1.41 ± 0.92
Liver	1.27 ± 0.18	1.09 ± 0.15	1.37 ± 0.08	1.81 ± 0.45	1.88 ± 0.44
Heart	0.44 ± 0.11	0.30 ± 0.12	0.39 ± 0.13	0.52 ± 0.19	0.63 ± 0.53
Kidneys	2.69 ± 0.25	2.83 ± 0.53	2.78 ± 0.30	2.93 ± 0.62	3.12 ± 0.97
Stomach	0.58 ± 0.19	0.51 ± 0.22	0.52 ± 0.12	0.67 ± 0.30	0.71 ± 0.13
Intestines	1.68 ± 0.13	1.98 ± 0.35	1.40 ± 0.17	1.96 ± 0.08	1.71 ± 0.36
Spleen	0.67 ± 0.12	0.73 ± 0.10	0.76 ± 0.07	0.98 ± 0.20	0.80 ± 0.17
Muscle	0.14 ± 0.04	0.12 ± 0.02	0.15 ± 0.02	0.22 ± 0.10	0.18 ± 0.11
Lungs	0.80 ± 0.15	0.93 ± 0.17	0.89 ± 0.10	1.33 ± 0.35	1.65 ± 1.02
Pancreas	0.55 ± 0.08	0.77 ± 0.09	0.60 ± 0.03	0.50 ± 0.10	0.45 ± 0.10
PC-3 Tumor	7.70 ± 0.89	11.57 ± 1.92	7.28 ± 1.00	3.57 ± 0.44	2.05 ± 0.53

¹ Mice pre-treated with Entresto^® (200 μL, 12 mg received per os 30 min before) as a sacubitrilat source. ² Mice co-injected with excess NT (100 µg for NTS₁R blockade). ³ Mice co-injected with excess [Tyr⁴]BBN (50 µg for GRPR blockade). ⁴ Mice co-injected with excess NT (100 µg) and [Tyr⁴]BBN (50 µg) for twin NTS₁R/GRPR in vivo blockade.