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. 2024 Sep 18;16(18):3146. doi: 10.3390/nu16183146

Figure 3.

Figure 3

Evaluation of gene expression in hMADS cells differentiated into white and brite adipocytes. (a) mRNA levels of UCP1, CPT1M, FABP4, PARG2, CIDEA, and MAPKAPK2. (b) mRNA levels of PLIN1, ATGL, HSL, RXRA, PPARG 1+2, ACOX1, COL1A, and ADRB3. Transfection of hMADS cells was conducted with 25 nM of the negative control (a scramble sequence) at day 10–12 of differentiation. Stimulation of the conversion of white adipocytes into brite adipocytes (browning process) was conducted from day 14 to day 18 of differentiation. Quantification of mRNA expression levels was conducted by qPCR in white and brite adipocytes. 36B4 was selected as housekeeping gene to normalize Cq values, which were expressed relative to the white negative control, applying the 2-ΔΔCt method. Results are depicted as the relative gene expression mean ± standard error of the mean (SEM) (n = 4–6). p-value: * p < 0.05, ** p < 0.01, *** p < 0.001, t (CIDEA) = 0.0813; t (MAPKAPK2) = 0.0551. Abbreviations: NC (negative control).