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. 2024 Sep 23;16(9):1238. doi: 10.3390/pharmaceutics16091238

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antiviral drug combination treatment using Loewe additivity fixed-dose assay. The analysis included the (A) combination of MBV + CCT-251921, (B) combination of MBV + MSC-2530818, and (C) combination of MBV + BI-1347. A modified protocol of the HCMV-GFP replication system was used, and cells were treated with either single compounds, compound combinations (MBV + CDK8 inhibitor CCT, MSC, or BI, at the concentration ratio of 1:1000), or a solvent control. Antiviral efficacy (mean of quadruplicate measurements of biological duplicates) was expressed as a percentage of the solvent control and analyzed using the CompuSyn software. Only experiments with an r value > 0.90 and EC50 values near previously determined concentrations were accepted.