FIG. 4.
Transfer of purified human B cells from healthy blood donors together with HBcAg into the spleens of conditioned NOD/SCID mice induces the production of HBcAg-binding human IgM. On day 0, 2 ×106 purified B cells were injected together with 10 μg of rHBcAg into the spleens of seven conditioned (300 rads of gamma radiation and 1 mg of TMβ1 administered intraperitoneally on day −1) NOD/SCID mice (●). Five mice received B cells (2 × 106 cells per mouse) without added HBcAg (▪). The percentage of animals in each group which displayed a positive signal in the anti-HBc IgM ELISA at dilutions of 1/10 to 1/4,000 is shown. The results shown were obtained 7 day after cell transfer.