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. 2005 Jun 6;102(24):8740–8745. doi: 10.1073/pnas.0503274102

Fig. 3.

Fig. 3.

In vitro detection of glutamate/aspartate in complex solutions. (A) Glutamate concentration in soy sauce, calf serum, and PC12 cell extract was determined by fitting a single-site binding isotherm. (B and C) Addition of external glutamate did not cause a signal change of FLIPE-10μcytosol (B) or FLIPE-1mcytosol (C) expressed in PC12 cells. B Lower and C Lower represent CFP (cyan) and Venus (yellow) emission intensity expressed relative to time point 0.