Table 1. FLIPE affinity mutants.

		Kd, M
Name of sensor	Mutation of ybeJ	Glutamate	Aspartate	Glutamine	Asparagine
FLIPE-600n	WT	6 × 10−7	6 × 10−6	1 × 10−4	2 × 10−4
FLIPE-10μ	A207R	1 × 10−5	6 × 10−5	1 × 10−3	n.d.
FLIPE-100μ	S118W	1 × 10−4	n.d.	n.d.	n.d.
FLIPE-1m	A207W	1 × 10−3	n.d.	n.d.	n.d.

Binding constants determined in vitro. n.d., not determined.