Table 3. BIAcore parameters for WT, LTM, and CBM clones.
scFv | kon (× 106 M-1·s-1) | Error | koff (× 10-5 s-1) | Error | kD, pM | Relative KD improvement |
---|---|---|---|---|---|---|
D2E7 | 1.12 | 0.02 | 107.000 | 0.1 | 955 ± 17 | |
A1 | 11.1 | 0.3 | 2.086 | 0.004 | 1.88 ± 0.05 | 500 |
cb2-44 | 5.8 | 0.4 | 0.637 | 0.01 | 1.10 ± 0.08 | 870 |
cb1-3 | 7.1 | 0.1 | 0.770 | 0.02 | 1.08 ± 0.02 | 870 |
cb2-6 | 4.0 | 0.9 | 0.0454 | 0.02 | 1.1 ± 0.3 | 870 |
A1 sequence has D2E7 mutations: CDRH1:D31Q, CDRH3:S99P, CDRL1:G28E.