Adoptively transferred B6.Sle3 DCs recreate Sle3-associated phenotypes. DCs cultured from B6 or B6.Sle3 BM (16–18 mice per strain, pooled from 4 independent experiments) were adoptively transferred into 2-month-old B6 mice on D0, D7, and D14, with coadministration of LPS on D1, D8, and D15. Plotted in A are the terminal splenic CD4/CD8 ratios in the host mice on D20 or D60; since the outcomes were similar at both time points, the results from D20 and D60 experiments have been pooled. Plotted in B are the serum IgG anti–single-stranded DNA Abs (left) and IgG anti–double-stranded DNA Abs (right) at serial time points during the 60-day study period, after the transfer of B6 or B6.Sle3 DCs, with coadministered LPS. All P values shown were computed by comparison of the B6.Sle3 values with the B6 control values, using the Student’s t test. The horizontal bars indicate the respective group means. The data shown were reproduced in 2 additional studies, as portrayed in Supplemental Figure 2.