Figure 5.
Multipotent capacity of cardiac precursors. (A) Oil red staining and RT-PCR analysis of PPARγ and lipoprotein lipase (LPL) expression in cultures of NMCs induced to differentiate into adipocytes. (B) Immunodetection of MyoD1 and RT-PCR analysis of MyoD1 expression in cultures of NMCs induced to differentiate into skeletal myoblasts. (C) Immunodetection of osteocalcin and RT-PCR analysis of osteocalcin expression in cultures of NMCs induced to differentiate into osteoblasts. Original magnification, ×20 (A and C), ×30 (C, inset), ×40 (B), ×63 (B, inset).