Figure 5.

Viral titers in nasal swabs and respiratory tissues. (a) Percentage of pigs that have become positive for virus shedding in nasal swabs collected at 0, 1, 3, and 5 d.p.i. (b) Virus titers in nasal swabs after challenge. (c) Virus titers in BALF and homogenized respiratory tissues at 5 d.p.i. Data are presented as TCID₅₀/mL for nasal swabs and BALF and TCID₅₀/g for respiratory tissues. Differences between treatments were analyzed by the Mantel–Cox log-rank test (a), Tukey’s multiple-comparisons test (b), or Dunn’s multiple-comparisons test (c). A statistically significant difference between two groups is indicated by different letters. Data are presented as the mean ± SEM. Symbols represent treatment groups (a) or individual pigs (b,c). SVNCh—sham vaccinated and not challenged, SV—sham vaccinated and challenged with CA04, αGC—vaccinated with MN08 mixed with 100 μg/kg of αGC and challenged with CA04, OIW—vaccinated with MN08 and the oil-in-water adjuvant Emulsigen-D and challenged with CA04, OIWαGC—vaccinated with combination of OIW and αGC and challenged with CA04.