Figure 8.

Nonsynonymous single-nucleotide variances detected in nasal swab samples collected at 5 d.p.i. (a) Total count of nonsynonymous SNVs per animal; (b) nonsynonymous SNVs located within epitopes of all CA04 proteins or (c) within HA and NA epitopes specifically. Differences between treatments were analyzed by Tukey’s multiple-comparisons test. Data are presented as the mean ± SEM. Symbols represent individual pigs. SVNCh—sham vaccinated and not challenged, SV—sham vaccinated and challenged with CA04, αGC—vaccinated with MN08 mixed with 100 μg/kg of αGC and challenged with CA04, OIW—vaccinated with MN08 and the oil-in-water adjuvant Emulsigen-D and challenged with CA04, OIWαGC—vaccinated with a combination of OIW and αGC and challenged with CA04.