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. 2005 Jun 9;115(7):1816–1827. doi: 10.1172/JCI24015

Figure 1.

Figure 1

Effect of chronic NO inhibition on B16F10 tumor vessels in the cranial window. (A) Representative microangiography images of B16F10 melanoma treated with D-NMMA (5 tumors, 5 locations each) or L-NMMA (6 tumors, 5 locations each). Scale bar: 100 μm. (B–D) Vessel parameters quantified by off-line analyses of the digitized microangiography images. Note that L-NMMA treatment resulted in significantly reduced vascular density (B), larger vessel diameter (C), and reduced branching (D). (E) Histological quantification of endothelial cell nuclei per vessel cross-section in B16F10 melanoma treated with D-NMMA (129 vessels in 3 tumors, 5 locations in each) or L-NMMA (83 vessels in 3 tumors, 5 locations in each). *P < 0.05 as compared with corresponding controls.