Figure 6.
Effect of DDD85646 on the expression of ISGs. A549 cells were seeded at 2 × 105 cells/well in an M12-well plate, infected with LCMV/GFP-P2A-NP (MOI 0.05), and treated with DDD85646 (5 or 10 µM) or with VC. At the indicated time points, total cellular RNA was isolated and analyzed by RT-qPCR (10 ng/sample; three technical replicates). The RT step was performed using random hexamers, followed by quantitative PCR with specific primers for ISGs MX1, or ISG15 and the cell housekeeping gene GAPDH. MX1 and ISG15 fold changes were normalized to GAPDH. The repeated-measures analysis of variance with mixed-effect analysis and Dunnet’s correction for multiple comparisons were used. Statistically significant values: ns p > 0.05, * p ≤ 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.