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Verification of the quantitative HIV-DNA PCR. (A) A total of 104 copies of HIV DNA in 8E5 cells were coamplified with 300, 1,500, 3,000, 6,000, or 30,000 copies of the competitor as shown in lanes 2 to 6, respectively. Lane 1 is the marker containing both wild-type (115 bp) and competitor (97 bp) amplicons, and lane 7 is blank. (B) Ratios of adjusted density of the competitor and wild-type bands plotted against the copy number of the competitor.
