FIG. 2.

Representative CCR5 RNA analysis by RT-PCR showing the purity of the cell preparations. The size of the amplification products is 189 bp. Lanes 1 and 2 show amplification from 200,000 cells of a CCR5-negative cell preparation and 60,000 cells of a CCR5-positive preparation, respectively. CCR5-positive (98% purity) and -negative (96% purity) cells highly purified by FACS were mixed to make cell preparations with various percentages of CCR5-positive cells in lanes 4 to 8. RNA from 100,000 cells was analyzed in each of these control lanes. The numbers below the lane are intensities of the bands calculated using Gel-Pro software.