FIG. 3.
The formation of multimers of K-bZIP requires amino acids within the proposed leucine zipper region. A. [35S]methionine-labeled His-K-bZIP, His-K-ΔZIP, and the Z-K hybrid series were generated in reticulocyte lysate. Products from the indicated translation reactions were analyzed by SDS-PAGE and quantitated by phosphorimaging. The migration of protein molecular weight markers (in kDa) is indicated on the left of each gel. B. In vitro association assays with GST and GST-K-bZIP agarose beads were undertaken with equal quantities of the indicated proteins. C. The products from the indicated translation reactions were analyzed by SDS-PAGE and quantitated by phosphorimaging.