Figure 9.

Western blot analysis of the impact of S272 phosphorylation on the proteasomal and autolysosomal degradation of p62. (a) Western blot analysis of p62 levels, following 5-day infection of ko-SQSTM1 cells with respective HCMV-p62-S272 strains (wt/D/A) using an m.o.i. of 0.2. Cells were treated with 10 µM of the proteasomal inhibitor MG132 18 h before sample collection. The results of one of two individual experiments are shown. (b) Western blot analysis of p62 levels applying 200 µM of the lysosomal inhibitor bafilomycin A1 (BafA1) 4 h before cell harvest, n = 1. (a,b) Cell lysates were probed with antibodies against p62, GAPDH (loading control), and LC3B.