TABLE 2.
ICAM-1 is essential for replication of all major group HRVs possessing a lysine in the VP1 HI loopa
| HRV serotypeb | Cell lysis
|
Inhibition of CPE by sICAM (EC50)c | ||||
|---|---|---|---|---|---|---|
| HeLa cells with:
|
COS-7 cells | |||||
| No addition (control) | Anti-ICAM R6.5 | MBP- V33333 | R6.5 and MBP- V33333 | |||
| 2 | + | + | − | − | + | NDd |
| 29 | + | + | − | − | + | ND |
| 23 | + | + | − | − | + | >100 |
| 25 | + | + | − | − | + | >100 |
| 8 | + | − | + | − | − | 3.1 |
| 18 | + | − | + | − | − | 1.8 |
| 24 | + | − | + | − | − | 1.1 |
| 58 | + | − | + | − | − | 1.2 |
| 40 | + | − | + | − | − | 2.1 |
| 54 | + | − | + | − | − | 3.0 |
| 56 | + | − | + | − | − | 0.6 |
| 85 | + | − | + | − | − | 6.8 |
| 98 | + | − | + | − | − | 0.1 |
| (14) | + | − | + | − | − | 5.5 |
| (16) | + | − | + | − | − | 0.4 |
| (8915e) | + | + | + | + | + | NDf |
a
HeLa cells grown in 48-well plates were challenged with the serotypes listed at a multiplicity of infection of 10 without addition (control) or in the presence of indicated additions and incubated for 3 days at 34°C. COS-7 cells were infected without addition of inhibitors. Viable cells remaining attached to the wells were stained with crystal violet. Clear wells indicating cell lysis were scored positive.
b
Boldface, minor group HRVs; HRVs in parentheses lack the lysine in their HI loop.
c
From reference 5.
d
ND, not determined.
f
Variant HRV8915 was more strongly neutralized by sICAM than the wt (24).