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(A) Coiled coil prediction of B5 proteins. The B5 amino acid sequence was used with COILS software (30, 31). Mutants L301P, L350P, L354P, L361P, and V364P changed leucine or valine to proline in the indicated positions. (B) Nucleotide sequence of the mutated region. The asterisks indicate mutation positions in the wild-type B5 gene. Numbers below correspond to nucleotides (nt) or amino acid positions from the initial nucleotide triplet of the B5 open reading frame. wt, wild type.
