FIG. 5.

Ad5/35GFP infection of CHO cells expressing truncated forms of CD46. (A) CHO cells were transfected with the indicated plasmids expressing either CCP1 (pI/4), CCP2 (pII/4), or the CCP1 and CCP2 domains together (pI+II/4), joined to a single immunoglobulin G-like domain of human CD4 protein. Transfected cells were stained with CD4-specific polyclonal antibody to detect levels of truncated CD46 protein expression. As a positive control, cells were transfected with full-length CD46-expressing plasmid (pCD46), and the levels of CD46 expression were determined using a rabbit polyclonal antibody (Ab) (indicated by the asterisk). Cells transfected with an unrelated plasmid were used as a control. (B) Efficiency of Ad5/35GFP infection of CHO cells transfected with the plasmids described for panel A. Note that only pCD46 and pI+II/4 plasmid transfection rendered CHO cells susceptible to virus infection. P values demonstrating statistically significant differences between levels of adenovirus infection of control cells and cells transfected with pCD46 or pI+II/4 plasmids are shown above the corresponding bars. n.s., not statistically significant. Error bars indicate standard deviations; n = 4.