Skip to main content
. 2001 Jul;75(14):6402–6409. doi: 10.1128/JVI.75.14.6402-6409.2001

FIG. 3.

FIG. 3

(a) Effects of z-VAD-FMK on DNA fragmentation in IBV-infected Vero cells. Cells were infected with 2 PFU of IBV per cell in the presence of DMSO (20 μl/ml) (lanes 4 and 5) or z-VAD-FMK (20 μg/ml) (lanes 2 and 3). Low-molecular-weight DNA was isolated from IBV-infected (I) Vero cells at 16 (lanes 2 and 4) and 36 (lanes 3 and 5) h postinfection and analyzed on a 2% agarose gel. The 1-kb ladder DNA markers (lane 1) were purchased from Gibco BRL (Life Technologies). (b) Effects of z-VAD-FMK on morphological changes of nuclei of IBV-infected Vero cells. Cells were infected with 2 PFU of IBV per cell in the presence of DMSO (20 μl/ml) or z-VAD-FMK (20 μg/ml). The nuclei were stained with Hochest 33342 at 36 h postinfection and viewed with a light microscope.