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. 2024 Aug 3;38(10):2183–2195. doi: 10.1038/s41375-024-02351-2

Fig. 5. In vitro evaluation of multiplex targeting by AdCAR-T.

Fig. 5

A Schematic depiction of the flow cytometry-based cytotoxicity assay: Molm13 wild type (WT), Molm13 CD33KO, Molm13 CD38KO, and Molm13 CD33/CD38 KO were mixed at a 1:1:1:1 ratio. The expression of CD33, CD38, and CD123 in individual cell populations is shown on the lower left. AdCAR-T cells were added at an E:T ratio of 1:1. LLE-aCD33, LLE-aCD38, or LLE-aCD123 as well as combinations thereof were added to reach a final concentration of 10 ng/mL. B UMAP representing batched surviving target cells after 48 h of incubation of all conditions (n = 3 each condition), based on the expression of CD33, CD38 and CD123, as determined by flow cytometry. From left to right, viable target cells for the indicated conditions plotted in color. From top to bottom, color-coded expression of CD33, CD38, and CD123.