TABLE 1.

Jagged1 cooperates with HPV-16 E6 or L83V E6 and E7 in transformation of HaCaT cells in soft agar colony formation assays^a

Vector	HaCaT-Neo	HaCaT-AcN1	HaCaT-Jagged1		HaCaT-Delta1
			Neo	MFng	Neo	MFng
Neo	4.6 ± 0.8	5 ± 0.5	5.3 ± 0.8	4 ± 0.5	4.3 ± 0.8	5 ± 0.5
E6 + E7	6 ± 0.5	27.6 ± 0.8	18.3 ± 1.4	10 ± 1.5	6.3 ± 0.8	5.6 ± 0.8
L83V E6 + E7	6 ± 0.57	56.3 ± 3.7	45 ± 2.3	27.3 ± 2.3	11.3 ± 0.88	13 ± 0.5

^aThe data represent mean ± standard error of the numbers of colonies generated by HaCaT cells expressing the mentioned combination of genes on soft agar assay in three independent experiments. The data were generated by microscopic counting of colonies in 20 random fields under a magnification of ×10 as described previously (33). HaCaT cells stably expressing mock vector (HaCaT Neo), activated Notch1 (HaCaT-AcN1), Jagged1 (HaCaT-Jagged1), and Delta1 (HaCaT-Delta1) are shown in boldface italic type, and transient infections of plasmids encoding E6, L83V E6 and E7, or mock neomycin resistance (Neo) alone or along with Manic Fringe (MFng) are shown in boldface roman type. Increases in colony numbers are shown in boldface roman type, and decreases in colony numbers are shown in boldface italic type.