FIG. 1.

The BRLF1 IE promoter (Rp) is highly methylated in latently infected cells. The methylation status of CpG sites in Rp in 5 EBV-positive cell lines (indicated on the left) was determined by sodium bisulfite treatment followed by PCR and sequencing. The position of each CpG site, represented by a diamond, with respect to the R transcription start site (+1) is indicated at the top. The percentage of clones with methylated CpG at each position is indicated on top of the diamond representing that CpG for each cell line analyzed; 10 clones for each cell were sequenced.